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Beamline B24, Diamond Light Source

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis
Nina Vyas 1, Mehdi Mahmud 2, Qianxi X Wang 2, A. Damien Walmsley 1
1School of Dentistry, University of Birmingham, 2Department of Mathematics, University of Birmingham

Cavitation microbubbles are imaged using a high-speed camera attached to a zoom lens. The experimental setup is explained, and image analysis is used to calculate the area of the cavitation. Image analysis is done using ImageJ.

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Biology

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Johannes Groen 1, Andrea Sorrentino 1, Lucía Aballe 1, Robert Oliete 1, Ricardo Valcárcel 1, Chidinma Okolo 2, Ilias Kounatidis 2, Maria Harkiolaki 2, Ana Joaquina Pérez-Berná 1, Eva Pereiro 1
1MISTRAL beamline, Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

Here, a protocol describing the sample preparation and data collection steps required in cryo soft X-ray tomography (SXT) to image the ultrastructure of whole cryo-preserved cells at a resolution of 25 nm half pitch, is presented.

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Biology

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
Nina Vyas *1, Nina Perry *1, Chidinma A. Okolo 1, Ilias Kounatidis 1, Thomas M. Fish 1, Kamal L. Nahas 1,2, Archana Jadhav 1, Mohamed A. Koronfel 1, Johannes Groen 3, Eva Pereiro 3, Ian M. Dobbie 4, Maria Harkiolaki 1
1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford

This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells.

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