Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron MicroscopyMasashi Yamaguchi 1, Azusa Takahashi-Nakaguchi 1, Katsuyuki Uematsu 2, Masaki Taguchi 2, Michiyo Sato-Okamoto 1, Hiroji Chibana 1
1Medical Mycology Research Center, Chiba University, 2Marine Works Japan Ltd.
Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.