Magna Graecia University of Catanzaro

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Licia E. Prestagiacomo¹, Caterina Gabriele¹, Paola Morelli², Maria Antonietta Rota³, Stefano Alba³, Giovanni Cuda¹, Rocco Damiano⁴, Marco Gaspari¹

¹Research Centre for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, ²Department of Health Science, Magna Graecia University of Catanzaro, ³Romolo Hospital, ⁴Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro

Here, we present an optimized on-filter digestion protocol with detailed information about the following: protein digestion, peptide purification and data independent acquisition analysis. This strategy is applied to the analysis of expressed prostatic secretions-urine samples and allows high proteome coverage and low missing value label-free profiling of the urinary proteome.