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Brigham and Women’s Hospital, Harvard Medical School

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Plasma Lithography Surface Patterning for Creation of Cell Networks
Michael Junkin 1, Siu Ling Leung 1, Yongliang Yang 1, Yi Lu 1, Justin Volmering 1, Pak Kin Wong 1,2
1Aerospace and Mechanical Engineering, University of Arizona , 2Biomedical Engineering IDP and BIO5 Institute, University of Arizona

A versatile plasma lithography technique has been developed to generate stable surface patterns for guiding cellular attachment. This technique can be applied to create cell networks including those that mimic natural tissues and has been used for studying several, distinct cell types.

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Medicine

Intraoperative Ultrasound in Spinal Surgery
Melissa M.J. Chua 1, Viren S. Vasudeva 1, Yi Lu 1
1Brigham and Women's Hospital, Harvard Medical School

Here, we present a protocol on the use of intraoperative ultrasound in spinal surgery, particularly in cases of intradural lesions and lesions in the ventral spinal canal when using a posterior approach.

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Immunology and Infection

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
Yi Lu 1,2,3, Xin-Yuan Fu 3, Yongliang Zhang 1,2
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore

Here, we present a protocol to differentiate murine granulocyte-macrophage-colony-stimulating-factor-producing T helper (THGM) cells from naive CD4+ T cells, including isolation of naive CD4+ T cells, differentiation of THGM, and analysis of differentiated THGM cells. This method can be applied to studies of the regulation and function of THGM cells.

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Neuroscience

Three-dimensional Navigation-guided, Prone, Single-position, Lateral Lumbar Interbody Fusion Technique
Samantha E. Hoffman 1, Saksham Gupta 1, Kevin Huang 1, Neil Klinger 1, Yi Lu 1
1Department of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School

The single-position, prone, lateral approach allows for both lateral lumbar interbody placement and direct posterior decompression with pedicle screw placement in one position.

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Biology

Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular Vesicles
Rachel M. E. Cahalane *1,2, Mandy E. Turner *1, Cassandra L. Clift 1, Mark C. Blaser 1, Gabrielle Bogut 1, Sydney Levy 1, Taku Kasai 1, Tom A. P. Driedonks 3,4, Esther N. M. Nolte-‘t Hoen 4, Masanori Aikawa 1,5, Sasha A. Singh 1,5, Elena Aikawa 1,5
1Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, 2Mechanobiology and Medical Device Research Group (MMDRG), Biomedical Engineering, College of Science and Engineering, University of Galway, 3Department of CDL Research, University Medical Center Utrecht, 4Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, 5Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School

A detailed protocol is provided for cleaning and re-using polycarbonate ultracentrifuge tubes to perform extracellular vesicle isolation suitable for proteomics experiments.

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