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Université de Paris, CNRS, Institut Jacques Monod

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Nuclear Migration in the Drosophila Oocyte
Maëlys Loh 1, Antoine Guichet 1, Fred Bernard 1
1Université de Paris, CNRS, Institut Jacques Monod

In Drosophila, the oocyte nucleus undergoes microtubule-dependent migration during oogenesis. Here, we describe a protocol that was developed to follow the migration by performing live imaging on egg chambers ex-vivo. Our procedure maintains egg chambers alive for 12 h to acquire multi-position 3D time-lapse movies using spinning-disk confocal microscopy.

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