Einstein College of Medicine / Montefiore Medical Center

5 ARTICLES PUBLISHED IN JoVE

Medicine

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

Allison S. Harney^1,2,3,4, Yarong Wang^1,3, John S. Condeelis^1,3,4, David Entenberg^1,3,4

¹Department of Anatomy & Structural Biology, Albert Einstein College of Medicine, ²Department of Radiology, Albert Einstein College of Medicine, ³Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, ⁴Integrated Imaging Program, Albert Einstein College of Medicine

This protocol describes the use of multiphoton microscopy to perform extended time-lapse imaging of multicellular interactions in real time, in vivo at single cell resolution.

Cancer Research

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window

Carolina Rodriguez-Tirado¹, Takanori Kitamura⁵, Yu Kato^1,2, Jeffery W. Pollard^1,2,5, John S. Condeelis^3,4, David Entenberg^3,4

¹Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, ²Department of Obstetrics/Gynecology and Woman’s Health, Albert Einstein College of Medicine, ³Department of Anatomy & Structural Biology, Albert Einstein College of Medicine, ⁴Gruss-Lipper Biophotonics Center Integrated Imaging Program, Albert Einstein College of Medicine, ⁵Medical Research Council Centre for Reproductive Health, Queen’s Medical Research Institute, University of Edinburgh

This protocol describes the use of multiphoton microscopy to perform long-term high-resolution, single cell imaging of the intact lung in real time using a vacuum stabilized imaging window.

Cancer Research

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

George S. Karagiannis^1,2,3, Jessica M. Pastoriza^1,2,4, Lucia Borriello^1,2, Rojin Jafari^1,2, Anouchka Coste^1,2,4, John S. Condeelis^1,2,3,4, Maja H. Oktay^1,2,3,5, David Entenberg^1,2,3

¹Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, ²Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, ³Integrated Imaging Program, Albert Einstein College of Medicine, ⁴Department of Surgery, Montefiore Medical Center, ⁵Department of Pathology, Montefiore Medical Center

We describe two methods for assessing transient vascular permeability associated with tumor microenvironment of metastasis (TMEM) doorway function and cancer cell intravasation using intravenous injection of high-molecular weight (155 kDa) dextran in mice. The methods include intravital imaging in live animals and fixed tissue analysis using immunofluorescence.

Cancer Research

A Permanent Window for Investigating Cancer Metastasis to the Lung

Lucia Borriello^*1,2, Brian Traub^*1,2,3, Anouchka Coste^1,2,3, Maja H. Oktay^1,2,4,5, David Entenberg^1,2,4

¹Department of Anatomy and Structural Biology, Einstein College of Medicine / Montefiore Medical Center, ²Gruss-Lipper Biophotonics Center, Einstein College of Medicine / Montefiore Medical Center, ³Department of Surgery, Einstein College of Medicine / Montefiore Medical Center, ⁴Integrated Imaging Program, Einstein College of Medicine / Montefiore Medical Cente, ⁵Department of Pathology, Einstein College of Medicine / Montefiore Medical Center

Here, we present a protocol for the surgical implantation of a permanently indwelling optical window for the murine thorax, which enables high-resolution, intravital imaging of the lung. The permanence of the window makes it well-suited to the study of dynamic cellular processes in the lung, especially those that are slowly evolving, such as metastatic progression of disseminated tumor cells.

Cancer Research

Stabilized Window for Intravital Imaging of the Murine Pancreas

Jakeb Petersen^*1,2,3, Wei Du^*4,5, Christian Adkisson^1,4, Claudia Gravekamp^6,7, Maja H. Oktay^2,3,4,7,8,9, John Condeelis^{1,3,4,7,8,9,10}, Nicole C. Panarelli^2,3,7, John C. McAuliffe^1,2,3,7,8, David Entenberg^2,3,4,7,8,9

We present a protocol for the surgical implantation of a stabilized indwelling optical window for subcellular-resolution imaging of the murine pancreas, allowing serial and longitudinal studies of the healthy and diseased pancreas.