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Skåne University Hospital

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Robotized Testing of Camera Positions to Determine Ideal Configuration for Stereo 3D Visualization of Open-Heart Surgery
Maj Stenmark 1,2, Edin Omerbašić 1,2, Måns Magnusson 1, Sanna Nordberg 3, Matilda Dahlström 3, Phan-Kiet Tran 1
1Cardiac Surgery Unit, Children’s Heart Centre, Skåne University Hospital, 2Department of Clinical Sciences, Lund University, 3Student at Lund University

The human depth perception of 3D stereo videos depends on the camera separation, point of convergence, distance to, and familiarity of the object. This paper presents a robotized method for rapid and reliable test data collection during live open-heart surgery to determine the ideal camera configuration.

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Medicine

Automated Vibratome Sectioning of Agarose-Embedded Lung Tissue for Multiplex Fluorescence Imaging
Qi Wang 1,2,3, Nicholas B. Bechet 1,2,3, Sandra Lindstedt 1,2,3,4
1Wallenberg Centre for Molecular Medicine, Lund University, 2Department of Clinical Sciences, Lund University, 3Lund Stem Cell Centre, Lund University, 4Department of Cardiothoracic Surgery and Transplantation, Skåne University Hospital

We have developed a tissue-processing technique utilizing a vibratome and agarose-embedded lung tissue to generate lung sections, thereby permitting the acquisition of high-resolution images of lung architecture. We employed immunofluorescence staining to observe spatial protein expression using specific lung structural markers.

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