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2 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts
Aleksandra Taran *1,2, Lilia Belikova (Shuvalova) *2,3, Svetlana Lavrushkina 1,2, Alexandra Bogomazova 3,4, Maria Lagarkova 3,4, Irina Alieva 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

This protocol is dedicated to the microtubule plus-end visualization by EB3 protein transfection to study their dynamic properties in primary cell culture. The protocol was implemented on human primary skin fibroblasts obtained from Huntington's disease patients.

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Developmental Biology

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
Artem Eremeev 1,3, Lilia Belikova 1,2, Evgeny Ruchko 1, Egor Volovikov 1,5, Olga Zubkova 1, Alexy Emelin 4, Roman Deev 4, Olga Lebedeva 1,3, Alexandra Bogomazova 1,3, Maria Lagarkova 1,3
1Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 4Department of Pathological Anatomy, North-Western State Medical University, 5Department of Biology, Lomonosov State University

Here we describe a protocol for generating brain organoids from human induced pluripotent stem cells (iPSCs). To obtain brain organoids in large quantities and of high quality, we use home-made mini bioreactors.

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