University of Washington, Tacoma

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

3D Reconstruction and Analysis of Thin Subcellular Neuronal Structures using Focused-Ion Beam Scanning Electron Microscopy Data

Jaclyn McCoy¹, Marc Nahmani¹

¹Division of Sciences & Mathematics, School of Interdisciplinary Arts & Sciences, University of Washington, Tacoma

A flexible methodological pipeline to identify, visualize, and quantify thin subcellular neuronal processes within focused ion beam scanning electron microscopy image volumes using user-friendly open-source software packages.