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Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, and Humboldt-Universität zu Berlin, and Berlin Institute of Health

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice
Simon H. Bayerl 1, Melina Nieminen-Kelhä 1, Thomas Broggini 2, Peter Vajkoczy 1, Vincent Prinz 1
1Department of Neurosurgery and Center for Stroke-research Berlin (CSB), Charité-Universitätsmedizin, 2Department of Physics, University of California San Diego

Surgical occlusion of a distal middle cerebral artery branch (MCAo) is a frequently used model in experimental stroke research. This manuscript describes the basic technique of permanent MCAo, combined with the insertion of a lateral cranial window, which offers the opportunity for longitudinal intravital microscopy in mice.

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Neuroscience

Endovascular Perforation Model for Subarachnoid Hemorrhage Combined with Magnetic Resonance Imaging (MRI)
Shuheng Liu *1, Katharina Tielking *1, Dario von Wedel 1, Melina Nieminen-Kelhä 1, Susanne Mueller 2,3, Philipp Boehm-Sturm 2,3, Peter Vajkoczy 1, Ran Xu 1
1Department of Neurosurgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, and Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Department of Experimental Neurology and Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3NeuroCure Cluster of Excellence and Charité Core Facility 7T Experimental MRIs, Charité - Universitätsmedizin Berlin

Here we present a standardized SAH mouse model, induced by endovascular filament perforation, combined with magnetic resonance imaging (MRI) 24 h after operation to ensure the correct bleeding site and exclude other relevant intracranial pathologies.

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