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Universidad Nacional de Rosario (UNR)

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
Victoria Lucia Alonso 1,2, Romina Manarin 1, Virginia Perdomo 1, Ernesto Gulin 3, Esteban Serra 1,2, Pamela Cribb 1,2
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner.

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Biology

Ultrastructural Expansion Microscopy in Three In Vitro Life Cycle Stages of Trypanosoma cruzi
María Azul de Hernández *1, Gonzalo Martinez Peralta *1,2, Rodrigo Vena 1,4, Victoria Lucia Alonso 1,3,4
1Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 2Area Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 3Area Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 4Unidad de Microscopía, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET

This study shows a detailed protocol to perform ultrastructure expansion microscopy in three in vitro life cycle stages of Trypanosoma cruzi, the pathogen responsible for Chagas disease. We include the optimized technique for cytoskeletal proteins and pan-proteome labeling.

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