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Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
Victoria Lucia Alonso 1,2, Romina Manarin 1, Virginia Perdomo 1, Ernesto Gulin 3, Esteban Serra 1,2, Pamela Cribb 1,2
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner.

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