the Sixth Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases

4 ARTICLES PUBLISHED IN JoVE

Environment

Choice and No-Choice Bioassays to Study the Pupation Preference and Emergence Success of Ectropis grisescens

Cai Wang¹, Huifang Wang¹, Tao Ma^1,2, Qiang Xiao³, Panrong Cao⁴, Xuan Chen⁵, Hongpeng Xiong¹, Wenquan Qin¹, Zhaohui Sun¹, Xiujun Wen¹

¹Guangdong Key Laboratory for Innovation Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, ²College of Agriculture, South China Agricultural University, ³Tea Research Institute, Chinese Academy of Agricultural Sciences, ⁴College of Horticulture, South China Agricultural University, ⁵College of Coast and Environment, Louisiana State University

Here, we present a protocol to investigate the pupation preference of mature larvae of Ectropis grisescens in response to soil factors (e.g., substrate type and moisture content) using choice bioassays. We also present a protocol of no-choice bioassays to determine the factors that affect the pupation behaviors and survivorship of E. grisescens.

Cancer Research

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

Shuang Chen¹, Andreas Giannakou¹, Jonathon Golas¹, Kenneth G. Geles¹

¹Oncology R&D group, Pfizer Worldwide Research and Development

An in vitro model system was developed to capture tissue architectural changes during lung squamous carcinoma (LUSC) progression in a 3-dimensional (3D) co-culture with cancer-associated fibroblasts (CAFs). This organoid system provides a unique platform to investigate the roles of diverse tumor cell-intrinsic and extrinsic changes that modulate the tumor phenotype.

Medicine

Application of Straight-needle, Three-tailed, Knot-free, Peritoneal Sutures in Laparoscopic Transabdominal Preperitoneal Hernia Repair

Ning Ma^*1, Zhen Zong^*2, Fu-xing Tang¹, En-min Huang¹, Tao Ma¹, Wei-sheng Yang¹, Jia-lin Chen¹, Shuang Chen¹, Tai-cheng Zhou¹

¹Department of Gastroenterological Surgery and Hernia Center, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, ²Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Nanchang University

Here, we present a protocol to introduce a new peritoneal suture method. This method is called straight-needle, three-tailed, knot-free suture, and we will outline the manufacturing method and clinical application of this suture in detail.

Medicine

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Ning Ma^*1, Dai Tang^*2, Fu-xing Tang¹, En-min Huang¹, Tao Ma¹, Wei-sheng Yang¹, Chuang-xiong Liu¹, Hao-nan Huang¹, Shuang Chen¹, Tai-cheng Zhou¹

¹Department of Gastroenterological Surgery and Hernia Center, The Sixth Affiliated Hospital, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangzhou, Sun Yat-sen University, ²Department of Gastrointestinal Surgery, the Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi)

Presented here is a method for improving the mesh placement in laparoscopic incisional hernia repair, which can shorten the time required for mesh fixation and reduce the occurrence of postoperative chronic pain.