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The Second Clinical College of Guangzhou University of Chinese Medicine

7 ARTICLES PUBLISHED IN JoVE

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Behavior

Bouncing Ball with a Uniformly Varying Velocity in a Metronome Synchronization Task
Yingyu Huang *1, Li Gu *1, Junkai Yang *1, Xiang Wu 1
1Department of Psychology, Sun Yat-Sen University

The purpose of this protocol is to introduce the application of a bouncing ball with a uniformly varying velocity in a metronome synchronization task.

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Medicine

A Detailed Protocol for Physiological Parameters Acquisition and Analysis in Neurosurgical Critical Patients
Xiang Wu 1,2, Guoyi Gao 1,2, Junfeng Feng 1,2, Qing Mao 1,2, Jiyao Jiang 1,2
1Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, 2Shanghai Institute of Head Trauma

Recently, we have developed a portable multimodality monitoring system for the monitoring of various physiological parameters in neurosurgical critical patients. Detailed protocols on how to use this multimodality monitoring system are presented here.

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Medicine

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Guihao Chen 1, Yuejin Yang 1, Chuansheng Xu 2, Shuo Gao 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

Here, we present a protocol that uses JC-1 dye to assess the mitochondrial membrane potential of cells after being exposed to hypoxia/reoxygenation with or without a protective agent.

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JoVE Journal

A 100 KW Class Applied-field Magnetoplasmadynamic Thruster
Baojun Wang 1, Haibin Tang 2, Yibai Wang 1, Chao Lu 1, Cheng Zhou 3, Yangyang Dong 1, Ge Wang 3, Yuntian Cong 3, Daniel Luu 4, Jinbin Cao 2
1Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Astronautics, Beihang University, 2Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Space and Environment, Beihang University, 3Beijng Institute of Control Engineering, 4School of Aerospace Engineering and Geodesy, University of Stuttgart

The goal of this protocol is to introduce the design of a 100 kW class applied-field magnetoplasmadynamic thruster and relevant experimental methods.

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Neuroscience

In Vivo Methods to Assess Retinal Ganglion Cell and Optic Nerve Function and Structure in Large Animals
Qian Ye *1, Zhonghao Yu *1, Tian Xia *1, Shengjian Lu 1, Jiaying Sun 1, Mengyun Li 1, Yu Xia 1, Si Zhang 1, Wencan Wu 1, Yikui Zhang 1
1The Eye Hospital, School of Ophthalmology & Optometry, Wenzhou Medical University

Here we demostrate several in vivo tests (flash visual evoked potential, pattern electroretinogram and optic coherence tomography) in goat and rhesus macaque to understand the structure and function of the optic nerve and its neurons.

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Medicine

Laparoscopic Radical Antegrade Modular Pancreatosplenectomy via Dorsal-Caudal Artery Approach for Pancreatic Neck-Body Cancer
Zhantao Shen 1,2, Guihao Chen 1,2, Chunbao Zhu 2, Xiang Wu 1,2, Yifeng Liu 1,2, Zhangyuanzhu Liu 1,2, Zhijian Tan 1,2, Xiaosheng Zhong 1,2
1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine

With advancements in laparoscopic techniques, laparoscopic radical antegrade modular pancreatosplenectomy (L-RAMPS) has been widely recognized. However, owing to several technical difficulties in this procedure, the artery-first approach in L-RAMPS still remains uncommon. Here, we developed the dorsal-caudal artery approach for L-RAMPS, which might be safe and beneficial for pancreatic neck tumors.

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Genetics

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model
Yu Xia *1,2, Jinlin Hu *3, Xiang Li 4, Shuang Zheng 4, Ge Wang 1,2, Songtao Tan 1,2, Zengxiao Zou 1,2, Qiong Ling 2,5, Fenghua Yang 4, Xiaoping Fan 1,2
1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation.

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