Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish LarvaeJie Ding *1, Rui Yan *1, Luna Wang 1, Qianlei Yang 1, Xiaoyun Zhang 1, Nan Jing 1, Yuanjie Wei 1, Hengdong Zhang 2,3, Yan An 1
1Department of Toxicology, School of Public Health, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Medical College of Soochow University, 2Department of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Control and Prevention, 3Jiangsu Preventive Medicine Association, Nanjing, China
Here, we have used alizarin red staining to show that lead acetate exposure causes a bone mass change in zebrafish larvae. This staining method can be adapted to the investigation of bone loss in zebrafish larvae loss induced by other hazardous toxicants.
