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University of Wrocław

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Gelatin Zymography to Detect Gelatinase Activity in Melanoma Cells
Ewa Mrówczyńska 1, Ewa Mazurkiewicz 1, Antonina Joanna Mazur 1
1Department of Cell Pathology, Faculty of Biotechnology, University of Wrocław

Metalloproteases (MMPs) are secreted by many cells, including malignant melanoma. MMP-mediated cleavage of extracellular matrix components leads to the increased invasive potential of these cells. Gelatin zymography, presented here, is a quantifying method for studying gelatinase activity manifested as a digested gelatin area on a polyacrylamide gel.

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Cancer Research

Isolation of Stably Transfected Melanoma Cell Clones
Ewa Mazurkiewicz 1, Ewa Mrówczyńska 1, Antonina Joanna Mazur 1
1Department of Cell Pathology, Faculty of Biotechnology, University of Wrocław

A protocol is described for isolating stably transfected melanoma cell clones using glass cylinders. We focus on practical advice that may be decisive for the success of the entire procedure.

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