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Texas Woman’s University

3 ARTICLES PUBLISHED IN JoVE

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Biology

Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI
Robbie D. Schultz 1, Tina L. Gumienny 1
1Department of Molecular and Cellular Medicine, Texas A&M University System Health Science Center, College of Medicine

We present a method to visualize cuticle in live C. elegans using the red fluorescent lipophilic dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), which is commonly used in C. elegans to visualize environmentally exposed neurons. With this optimized protocol, alae and annular cuticular structures are stained by DiI and observed using compound microscopy.

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Biology

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
Katherine K. Beifuss 1, Tina L. Gumienny 1
1Department of Molecular and Cellular Medicine, Texas A&M University System Health Science Center

We describe a sensitized method to identify postembryonic regulators of protein expression and localization in C. elegans using an RNAi-based genomic screen and an integrated transgene that expresses a functional, fluorescently tagged protein.

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Biology

Small-Scale Extraction of Caenorhabditis elegans Genomic DNA
Bhoomi Madhu 1, Mohammed Farhan Lakdawala 1, Tina L. Gumienny 1
1Department of Biology, Texas Woman’s University

Presented here is a description of the straightforward and relatively rapid isolation of Caenorhabditis elegans genomic DNA from one or a few animals using a commercially available tissue kit. The resulting gDNA preparation is a suitable template for PCR.

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