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Chinese Academy of Medical Science and Peking Union Medical College

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Isolation and Identification of Porcine Bone Marrow Mesenchymal Stem Cells and their Derived Extracellular Vesicles
Wenyang Jiang 1, Yongchun Cui 2, Xiaosong Li 1, Yuyan Xiong 1, Guangxin Yue 2, Yuejin Yang 3
1State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Science and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Center for Cardiovascular Experimental Study and Evaluation, National Center for Cardiovascular Diseases, Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials, Animal Experimental Center, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 3State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Science and Peking Union Medical College

This article elaborates a method to isolate and identify porcine bone marrow mesenchymal stem cells (pBM-MSCs) and extracellular vesicles (EVs) derived from them, providing a methodological basis for the pre-clinical evaluation of transplantation efficacy of BM-MSCs and their derived EVs.

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Biology

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
Dingyun Song 1,2, Yicong Chen 1,3, Xin Wang 1,4, Xueying Chen 1,2, Shuwei Gao 1,3, Wenxiu Xu 1,3, Shengnan Yang 5, Zai Wang 6, Liang Peng 7, Huaping Dai 1,2
1Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, National Clinical Research Center for Respiratory Diseases, Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, 2Graduate School of Peking Union Medical College, Chinese Academy of Medical Science and Peking Union Medical College, 3Capital Medical University, 4Beijing University of Chinese Medicine, 5Tianjin Chest Hospital, 6Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, 7Beijing Key Laboratory for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital

Various animal models of pulmonary fibrosis have been established using bleomycin to clarify the pathogenesis of pulmonary fibrosis and find new drug targets. However, most pulmonary fibrosis models targeting lung tissue have uneven drug administration. Here, we propose a model of uniform pulmonary fibrosis induced by nasal bleomycin nebulization.

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