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Norris Cotton Cancer Center

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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
Kali A. Smolen 1, Arminja N. Kettenbach 1,2
1Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, 2Norris Cotton Cancer Center

Here, we present a protocol for the enrichment of endogenous phosphoprotein phosphatases and their interacting proteins from cells and tissues and their identification and quantification by mass spectrometry-based proteomics.

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