Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based ImmunoassaysJosé A. Hernández-Ortiz 1, Pablo E. Guevara-Pantoja 1, Mariana Andrade-Medina 1,2, Mauricio Carrillo-Tripp 1,2, Gabriel A. Caballero-Robledo 1
1Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), 2Biomolecular Diversity Laboratory, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav)
Microfluidics is a powerful tool for the development of diagnostic tests. However, expensive equipment and materials, as well as laborious fabrication and handling techniques, are often required. Here, we detail the fabrication protocol of an acrylic microfluidic device for magnetic micro- and nanoparticle-based immunoassays in a low-cost and simple-to-use setting.