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Shandong Provincial Hospital Affiliated to Shandong First Medical University

5 ARTICLES PUBLISHED IN JoVE

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Biology

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
Hui Zhang 1,2, Niko G. Gubernator 3,4, Minerva Yue 1, Roland G. W. Staal 1, Eugene V. Mosharov 1, Daniela Pereira 1, Vojtech Balsanek 3, Paul A. Vadola 3, Bipasha Mukherjee 5, Robert H. Edwards 5, David Sulzer 1,2,6, Dalibor Sames 3
1Departments of Neurology, Columbia University, 2Departments of Psychiatry and Pharmacology, Columbia University, 3Department of Chemistry, Columbia University, 4eMolecules, Inc., 5Departments of Neurology and Physiology, University of California School of Medicine, San Francisco, 6Division of Molecular Therapeutics, New York Psychiatric Institute

A new means to measure neurotransmission optically using fluorescent dopamine analogs.

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Cancer Research

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
Xiaoyu An *1,2, Xuesong Ouyang *1, Hui Zhang 1, Tingting Li 1, Yu-yang Huang 1, Zhiyuan Li 1, Demin Zhou 2, Qi-Xang Li 1,2
1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University

The experimental procedure on the immunophenotyping of murine orthotopic PDAC homografts aims at profiling the tumor immuno-microenvironment. Tumors are orthotopically implanted via surgery. Tumors of 200–600 mm3 in size were harvested and dissociated to prepare single-cell suspensions, followed by multi-immune marker FACS analysis using different fluorescently-labeled antibodies.

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Neuroscience

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice
Lianteng Zhi 1, Jingyu Zhao 1, David Jaffe 1, Yuanxin Chen 1, Ninghan Wang 1,2, Qi Qin 1, Erin L. Seifert 3, Chenjian Li 4, Hui Zhang 1
1Department of Neuroscience, Thomas Jefferson University, 2School of Biomedical Engineering, Drexel University, 3Department of Pathology, MitoCare Center, Thomas Jefferson University, 4School of Life Sciences, Peking University

Oxygen consumption rate (OCR) is a common proxy for mitochondrial function and can be used to study different disease models. We developed a new method using a Seahorse XF analyzer to directly measure the OCR in acute striatal slices from adult mice that is more physiologically relevant than other methods.

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Medicine

Extraperitoneal Laparoscopic Modified Y-V Plasty for the Treatment of Refractory Bladder Neck Contracture
Zhenjie Zang 1, Dingchang Shao 1, Hui Zhang 2, Dingqi Sun 2, Tongxiang Diao 2, Keqin Zhang 2, Qiang Fu 1,2
1Department of Urology, Shandong Provincial Hospital, Shandong University, 2Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University

Here, we describe a modified surgical procedure for refractory bladder neck contracture that is associated with easy manipulation, optimum intraoperative exposure, and minimal invasiveness.

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Biology

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model
Yanjing Guo 1,2,3, Wei Li 2,3, Yinghan Wang 2
1The Affiliated Hospital of Qingdao University, 2Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, 3Department of Anesthesiology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University

Follicle-stimulating hormone (FSH) in various extragonadal tissues and organs is associated with the pathogenesis of multiple diseases. The ovariectomized and FSH-treated mouse model (OVF) can be used to explore the extragonadal actions of FSH.

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