Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow CytometryRebecca Lees *1, Robert Tempest *1, Alice Law *1, Dimitri Aubert *1, Owen G. Davies *2, Soraya Williams *2, Nick Peake *3, Ben Peacock *1
1NanoFCM Co., Ltd, 2School of Sport, Exercise and Health Sciences, Loughborough University, 3Biomolecular Sciences Research Centre, Sheffield Hallam University
The latest generation of EV characterization tools are capable of single EV analysis across multiple parameters simultaneously. Nano-flow cytometry measures all biological particles larger than 45 nm without labeling and identifies specific characteristics of subpopulations by a variety of fluorescent labeling techniques.