Spatiotemporal Analysis of Microglial Ca2+ Activity at Single-Cell ResolutionHiroshi Horiuchi 1,2, Dennis Lawrence Cheung 1, Junichi Nabekura 1,2
1Division of Homeostatic Development, National Institute for Physiological Sciences, 2Department of Physiological Sciences, SOKENDAI: the Graduate University for Advanced Studies
In this paper, we describe a protocol for in vivo imaging of microglial Ca2+ activity and subsequent analysis of its spatiotemporal dynamics. This method enables thorough characterization of how microglia respond to changes in the brain environment, appropriately capturing the fine spatiotemporal scales at which such events occur.
