VIB-UGent Center for Inflammation Research

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Josephine Nemegeer^1,2, Kelly Lemeire^1,2, Peter Vandenabeele^1,2, Jonathan Maelfait^1,2

¹VIB-UGent Center for Inflammation Research, ²Department of Biomedical Molecular Biology, Ghent University

Tyramide signal amplification during immunofluorescent staining enables the sensitive detection of phosphorylated RIPK3 and MLKL during ZBP1-induced necroptosis after HSV-1 infection.