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Chinese Academy of Agricultural Sciences, Ministry of Agriculture

5 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats
Shihai Jiang *1, Xiaoyou Yi *3, Yuansen Luo 2, Dongjie Yu 1, Yuangao Liu 1, Fei Zhang 1,4, Lei Zhu 2, Kun Wang 1
1Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 2Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 3Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, 4Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University

Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.

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Medicine

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis
Kaiyue Zhang 1, Chen Wang 1, Ran Wang 2, Shang Chen 1, Zongjin Li 1
1Nankai University School of Medicine, 2State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University

This protocol describes the establishment of a tumor-bearing mouse model to monitor tumor progression and angiogenesis in real-time by dual bioluminescence imaging.

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Medicine

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes
Yun Liu *1, Yin Liang *1, Mengxue Wang *1, Chen Wang 1, Heng Wei 2, Keiji Naruse 1, Ken Takahashi 1
1Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.

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Immunology and Infection

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
Marieke de Korte 1,2, Armand Keating 1,2,4, Chen Wang 1,3
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 2Krembil Research Institute, Toronto Western Hospital, University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, 4Medical Oncology and Hematology, Princess Margaret Cancer Centre

This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.

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Environment

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples
Weiting Jiao 1,2, Tingting Shen 2, Luyao Wang 1, Lei Zhu 1,3, Qing X. Li 4, Chen Wang 3, Hongping Chen 3, Rimao Hua 1, Xiangwei Wu 1
1Key Laboratory of Agri-food Safety of Anhui Province, School of Resource & Environment, Anhui Agricultural University, 2State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, 3Key Laboratory of Tea Quality and Safety & Risk Assessment, Tea Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, 4Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa

The present protocol describes the contamination of pyrrolizidine alkaloids (PAs) in tea samples from PA-producing weeds in tea gardens.

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