Key Laboratory of Ethnomedicine (Minzu University of China)

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

Qiao Li¹, Lin Lu¹, Huimin Tao¹, Carolyn Xue¹, Seagal Teitz-Tennenbaum², John H. Owen³, Jeffrey S Moyer³, Mark E.P. Prince³, Alfred E. Chang¹, Max S. Wicha²

¹Department of Surgery, University of Michigan, ²Department of Internal Medicine, University of Michigan, ³Department of Otolaryngology, University of Michigan

Evaluated in syngeneic immunocompetent hosts, cancer stem cell (CSC) based dendritic cell (DC) vaccine demonstrated significantly higher antitumor immunity than traditional DC vaccines pulsed with heterogeneous bulk tumor cells.

Biology

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Chang Li^*1,2, Zhu Hui Liu^*1,2, Jia Wei Chen^1,2, Xin Yi Shu^1,2, Ying Shen¹, Feng Hua Ding¹, Rui Yan Zhang¹, Wei Feng Shen¹, Lin Lu¹, Xiao Qun Wang¹

¹Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, ²Institute of Cardiovascular Diseases, Shanghai Jiao Tong University

Here, we present a protocol for immunofluorescence staining to observe the endothelial cells of the mouse aorta directly. This technique is useful when studying the cellular and molecular phenotype of endothelial cells in different flow patterns and in the development of atherosclerosis.

Bioengineering

Co-Fermentation of Marsdenia tenacissima with Ganoderma lucidum and Anti-Lung Cancer Effect of the Fermentation Products

Runtian Li^1,2, Zhiguang Zhang^1,2, Siqi Wang^1,2, Jiaoneng Yu^1,2, Xinxin Su^1,2, Lin Lu^1,2, Tongxiang Liu^1,2

¹School of Pharmacy, Minzu University of China, ²Ministry of Education, Key Laboratory of Ethnomedicine (Minzu University of China)

Here, we describe a protocol to establish a systematic and comprehensive co-fermentation system to produce biotransformation products rich in pungent saponins by using Marsdenia tenacissima (Roxb.) Wight et Arn. (MT) as a fermentation medium for Ganoderma lucidum. This will serve as a methodological reference for the development of other ethnic drugs.