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Université de Rennes

3 ARTICLES PUBLISHED IN JoVE

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Biology

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
Eloïse Mussard 1,2, Corinne Lencina 1, Gaëlle Boudry 3, Caroline S. Achard 2, Christian Klotz 4, Sylvie Combes 1, Martin Beaumont 1
1GenPhySE, Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM, Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute

Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.

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Developmental Biology

Studying Muscle Transcriptional Dynamics at Single-molecule Scales in Drosophila
Emma Leroux 1, Nourhene Ammar 1, Savannah Moinet 1, Thierry Pecot 2, Hadi Boukhatmi 1
1Institut de Génétique et Développement de Rennes (IGDR), UMR6290 CNRS - Université de Rennes, 2BIOSIT, UAR 3480 US 018, Université de Rennes

Drosophila is a well-established model for studying key molecules that regulate myogenesis. However, current methods are insufficient to determine mRNA transcriptional dynamics and spatial distribution within syncytia. To address this limitation, we optimized an RNA fluorescence in situ hybridization method allowing the detection and quantification of mRNAs at a single-molecule scale.

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Medicine

A Swine Burn Model for Investigating the Healing Process in Multiple Depth Burn Wounds
Yanis Berkane 1,2,3,4, Rohil Jain 2,5, Emmanuella O. Ajenu *2,5, Austin A. Shamlou *1,2, Khanh Nguyen 2,5, Michelle McCarthy 1,2,5, Basak E. Uygun 2,5, Alexandre G. Lellouch 1,2,6, Curtis L. Cetrulo Jr. 1,2, Korkut Uygun 2,5, Mark A. Randolph 1,2, Shannon N. Tessier 2,5
1Vascularized Composite Allotransplantation Laboratory, Massachusetts General Hospital, Harvard Medical School, 2Shriners Children’s Boston, 3Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University, 4SITI Laboratory, UMR, INSERM, Université de Rennes, 5Center for Engineering in Medicine and Surgery, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, 6Innovative Therapies in Haemostasis, INSERM UMR-S, University of Paris

This protocol describes a reproducible multi-depth burn wound model in a Yucatan minipigs.

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