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1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
Alan Tin Lun Lam 1, Premkumar Jayaraman 2, Abigail Becker 3, Ryan Lim 2, Kim Leng Teo 1, Jacqueline Ng 2, Steve Oh 1
1Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific

Here, we present a protocol to harvest adherent cells from multi-layered flasks in a closed semi-automated manner using a counterflow centrifugation system. This protocol can be applied for harvesting both adherent and suspension cells from other cell expansion platforms with few modifications to the existing steps.

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