Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume ImagingAnna M. Steyer 1,2, Torben Ruhwedel 1, Wiebke Möbius 1,2
1Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine, 2Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
Here we present a protocol for combining two sample processing techniques, high-pressure freezing and microwave-assisted sample processing, followed by minimal resin embedding for acquiring data with a focused ion beam scanning electron microscope (FIB-SEM). This is demonstrated using a mouse tibial nerve sample and Caenorhabditis elegans.
