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Center of Biomedical Investigation Network for Neurodegenerative Diseases (CIBERNED)

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma
Natalia Valle-Tamayo 1,2, Rocío Pérez-González 2,3, Gemma Chiva-Blanch 4,5, Olivia Belbin 1,2, Sara Serrano-Requena 1,2, Sònia Sirisi 1,2, Alba Cervantes González 1,2, Oriol Giró 4,5, Érika Sánchez-Aced 1,2, Oriol Dols-Icardo 1,2, Daniel Alcolea 1,2, María Carmona-Iragui 1,2, Amanda Jimenez 4,5, Alberto Lleó 1,2, Juan Fortea 1,2, M. Florencia Iulita 1,2
1Sant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau, Universitat Autònoma de Barcelona, 2Center of Biomedical Investigation Network for Neurodegenerative Diseases (CIBERNED), 3Movement Disorders Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, 4Department of Endocrinology and Nutrition, Hospital Clínic de Barcelona, August Pi i Sunyer Biomedical Research Institute - IDIBAPS, 5Spanish Biomedical Research Network in Physiopathology of Obesity and Nutrition (CIBEROBN)

This protocol describes the enrichment of astrocyte-derived extracellular vesicles (ADEVs) from human plasma. It is based on the separation of EVs by polymer precipitation, followed by ACSA-1 based immunocapture of ADEVs. Analysis of ADEVs may offer clues to study changes in inflammatory pathways of living patients, non-invasively by liquid biopsy.

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