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5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
Dustin R. Morado 1, Bo Hu 1, Jun Liu 1
1Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston

We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.

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JoVE Core

Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes
Du Zhou 1, Xi Yuan 1, Haoxiang Gao 1, Ailing Wang 1, Jun Liu 1, Omer El Fakir 1, Denis J. Politis 1, Liliang Wang 1, Jianguo Lin 1
1Department of Mechanical Engineering, Imperial College London

The following paper presents a novel FE simulation technique (KBC-FE), which reduces computational cost by performing simulations on a cloud computing environment, through the application of individual modules. Moreover, it establishes a seamless collaborative network between world leading scientists, enabling the integration of cutting edge knowledge modules into FE simulations.

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Developmental Biology

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
Shen Zhang 1, Yonggen Wu 1, Yimin Weng 2, Zhihui Xu 1, Wenmin Chen 3, Dahan Zheng 4, Wei Lin 5, Jun Liu 6, Ying Zhou 1,7
1Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, 2Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, 3Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, 4School of Laboratory Medicine and Life Science, Wenzhou Medical University, 5School of Pharmaceutical Science, Wenzhou Medical University, 6Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, 7Department of Histology and Embryology, Wenzhou Medical University

A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.

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Bioengineering

Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement
Jun Liu 1, John Wilson 2, Claire Davis 1, Anthony Peyton 2
1Advanced Steel Research Centre, Warwick Manufacturing Group, University of Warwick, 2School of Electrical and Electronic Engineering, University of Manchester

This paper elaborates the sample and sensor preparation procedures and the protocols for using the test rig particularly for dynamic domain imaging with in situ BH measurements in order to achieve optimal domain pattern quality and accurate BH measurements.

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Immunology and Infection

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
David Chetrit 1, Donghyun Park 1,2, Bo Hu 3, Jun Liu 1,2, Craig R. Roy 1
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, 2Microbial Sciences Institute, Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston

Imaging of bacterial cells is an emerging systems biology approach focused on defining static and dynamic processes that dictate the function of large macromolecular machines. Here, integration of quantitative live cell imaging and cryo-electron tomography is used to study Legionella pneumophila type IV secretion system architecture and functions.

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