Goethe University Hospital Frankfurt

2 ARTICLES PUBLISHED IN JoVE

Medicine

The CYP2D6 Animal Model: How to Induce Autoimmune Hepatitis in Mice

Edith Hintermann¹, Janine Ehser¹, Urs Christen¹

¹Pharmazentrum Frankfurt / ZAFES, Goethe University Hospital Frankfurt

Infection of mice with an Adenovirus expressing the major human autoantigen cytochrome P450 2D6 (hCYP2D6) recognized by sera of patients suffering from type 2 autoimmune hepatitis results in a persistent form of autoimmune-mediated liver disease characterized by extensive hepatitis, fibrosis and generation of a CYP2D6-specific immune response.

Immunology and Infection

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Julia Barthelmes^*1, Nadja Tafferner^*2, Jennifer Kurz², Natasja de Bruin², Michael J. Parnham², Gerd Geisslinger¹, Susanne Schiffmann²

¹Institute of Clinical Pharmacology, Goethe University Hospital Frankfurt, ²Project Group for Translational Medicine & Pharmacology, Fraunhofer IME

This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.