A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord InjuryHussein Atta *1,2, Ali Hashemi Gheinani *1,2, Amanda Wacker 1, Yaser Heshmati 3,4,5, Alex Bigger-Allen 1,6, George Lambrinos 1,2, Yao Gao 2,7, Diane R. Bielenberg 2,7, Rosalyn M. Adam 1,2
1Department of Urology, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School, 3Division of Hematology/Oncology, Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, 7Vascular Biology Program, Boston Children's Hospital
The goal of this protocol is to apply an optimized tissue dissociation protocol to a mouse model of spinal cord injury and validate the approach for single cell analysis by flow cytometry.
