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Harvard Medical School, Massachusetts General Hospital

9 ARTICLES PUBLISHED IN JoVE

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Biology

PDMS Device Fabrication and Surface Modification
Kenneth Kotz 1, Xuanhong Cheng 1, Mehmet Toner 1
1Havard Medical School, MGH - Massachusetts General Hospital

PDMS Device Fabrication and Surface Modification

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Biology

Cell Capture Using a Microfluidic Device
Kenneth Kotz 1, Xuanhong Cheng 1, Mehmet Toner 1
1Havard Medical School, MGH - Massachusetts General Hospital

Cell Capture Using a Microfluidic Device

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Biology

Development of New Therapeutic Applications Using Microfluidics
Mehmet Toner 1
1Havard Medical School, MGH - Massachusetts General Hospital

Development of New Therapeutic Applications Using Microfluidics

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Bioengineering

Decellularization and Recellularization of Whole Livers
Basak E. Uygun 1, Gavrielle Price 1, Nima Saeidi 1, Maria-Louisa Izamis 1, Tim Berendsen 1, Martin Yarmush 1, Korkut Uygun 1
1Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Shriners Hospitals for Children

Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.

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Medicine

Ex Situ Normothermic Machine Perfusion of Donor Livers
Negin Karimian 1,2, Alix P.M. Matton 1,2, Andrie C. Westerkamp 1,2, Laura C. Burlage 1,2, Sanna op den Dries 1,2, Henri G.D. Leuvenink 2, Ton Lisman 1,2, Korkut Uygun 3, James F. Markmann 4, Robert J. Porte 1,2
1Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, University of Groningen, University Medical Center Groningen, 2Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, 3Center of Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, 4Division of Transplantation, Department of Surgery, Massachusetts General Hospital, Harvard Medical School

Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.

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Medicine

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
Bote G. Bruinsma *1, James H. Avruch *2, Pepijn D. Weeder 1, Gautham V. Sridharan 1, Basak E. Uygun 1, Negin G. Karimian 1, Robert J. Porte 3, James F. Markmann 2, Heidi Yeh 2, Korkut Uygun 1
1Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, 2Transplant Center, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, 3Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University Medical Center Groningen, University of Groningen

We describe a method of ex vivo machine perfusion of human liver grafts at subnormothermic temperature (21 °C).

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Bioengineering

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
Kim V. Berghaus 1, Seok H. Yun 2,3, Giuliano Scarcelli 1
1Fischell Department of Bioengineering, University of Maryland, 2Wellman Center for Photomedicine, Harvard Medical School, Massachusetts General Hospital, 3The Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology

Here we present a protocol to build a rapid Brillouin spectrometer. Cascading virtually imaged phase array (VIPA) etalons achieve a measurement speed more than 1,000 times faster than traditional scanning Fabry-Perot spectrometers. This improvement provides the means for Brillouin analysis of tissue and biomaterials at low power levels in vivo.

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Bioengineering

Bulk Droplet Vitrification for Primary Hepatocyte Preservation
Reinier J. de Vries 1,2,3, Peony D. Banik 1,2, Sonal Nagpal 1,2, Lindong Weng 1,2, Sinan Ozer 1,2, Thomas M. van Gulik 3, Mehmet Toner 1,2, Shannon N. Tessier 1,2, Korkut Uygun 1,2
1Center for Engineering in Medicine, Harvard Medical School, Massachusetts General Hospital, 2Shriners Hospitals for Children, Boston, 3Department of Surgery, University of Amsterdam

This manuscript describes an ice-free cryopreservation method for large quantities of rat hepatocytes whereby primary cells are pre-incubated with cryoprotective agents at a low concentration and vitrified in large droplets.

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Immunology and Infection

Partial Heterotopic Hindlimb Transplantation Model in Rats
Marion Goutard 1,2,3, Mark A. Randolph 1,2,3, Corentin B. Taveau 1,2,3,4, Elise Lupon 1,2,3, Laurent Lantieri 4, Korkut Uygun 1,2,3, Curtis L. Cetrulo Jr. *1,2,3, Alexandre G. Lellouch *1,2,3,4
1Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Harvard Medical School, 2Vascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, 3Shriners Hospital for Children, 4Service de Chirurgie Plastique, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris (APHP), Université de Paris

This paper presents a partial heterotopic osteomyocutaneous flap transplantation protocol in rats and its potential outcomes in the mid-term follow-up.

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