HistoRx Inc.

1 ARTICLES PUBLISHED IN JoVE

Medicine

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Dana Faratian¹, Jason Christiansen², Mark Gustavson², Christine Jones², Christopher Scott², InHwa Um¹, David J. Harrison¹

¹Division of Pathology, University of Edinburgh, ²HistoRx Inc.

Here we describe a method to quantify molecular heterogeneity in histological sections of tumor material using quantitative immunofluorescence, image analysis, and a statistical measure of heterogeneity. The method is intended for use in clinical biomarker development and analysis.