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INOVA Diagnostics, Inc.

2 ARTICLES PUBLISHED IN JoVE

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Biology

Western Blotting: Sample Preparation to Detection
Anna Eslami 1, Jesse Lujan 1
1Research and Development, EMD Chemicals Inc.

Western blotting is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract.

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Biology

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Carol Buchner 1, Cassandra Bryant 2, Anna Eslami 3, Gabriella Lakos 4
1IFA Development Manager, INOVA Diagnostics, Inc., 2IFA Development, INOVA Diagnostics, Inc., 3Product Manager, Rheumatology, INOVA Diagnostics, Inc., 4Medical Director, INOVA Diagnostics, Inc.

Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.

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