A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegansJyoti Dubey 1,2,3,4, Sudip Mondal 1,5, Sandhya P. Koushika 2
1National Centre for Biological Sciences, TIFR, 2Department of Biological Sciences, TIFR, 3InSTEM, 4Manipal Academy of Higher Education, 5Department of Mechanical Engineering, The University of Texas at Austin
The protocol describes a simple microfluidic chip design and microfabrication methodology used to grow C. elegans in presence of a continuous food supply for up to 36 h. The growth and imaging device also enables intermittent long-term high-resolution imaging of cellular and sub-cellular processes during development for several days.
