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Indiana University Indianapolis

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
Madhumita Sridharan 1, Tripthi Battapadi 1, Lata Balakrishnan 1
1Department of Biology, Indiana University Indianapolis

This article provides a procedure for the affinity purification of a human recombinant protein, flap endonuclease 1 (FEN1), which has been labeled with a 6X-histidine tag. The protocol involves the utilization of two distinct immobilized metal ion columns for the purification of the tagged protein.

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