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Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France.

2 ARTICLES PUBLISHED IN JoVE

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Behavior

Recording Mouse Ultrasonic Vocalizations to Evaluate Social Communication
Allain-Thibeault Ferhat 1, Nicolas Torquet 2, Anne-Marie Le Sourd 1, Fabrice de Chaumont 3, Jean-Christophe Olivo-Marin 3, Philippe Faure 2, Thomas Bourgeron 1, Elodie Ey 1
1Human Genetics and Cognitive Functions, University Paris Diderot, CNRS UMR 3571, Institut Pasteur, 2Neurophysiology and Behavior, University Pierre et Marie Curie Paris 6, CNRS UMR 7102, 3Bio Image Analysis, CNRS URA 2582, Institut Pasteur

Mouse ultrasonic vocalizations are used as proxies to model the genetic bases of vocal communication deficits in mouse models for neuropsychiatric disorders. The present protocol describes three experimental contexts that reliably elicit ultrasonic vocalizations from pups (throughout development) and adult mice (same-sex interactions, male-estrus female interactions).

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Bioengineering

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
Géraud Heckler 1, Christelle Aigueperse 1, Liza Hettal 1, Quentin Thuillier 1, Fabrice de Chaumont 2, Stéphane Dallongeville 2, Isabelle Behm-Ansmant 1
1Université de Lorraine, CNRS, IMoPA F54000 Nancy France, 2Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France.

We present a freely available workflow built for rapid exploration and accurate analysis of cellular bodies in specific cell compartments in fluorescence microscopy images. This user-friendly workflow is designed on the open-source software Icy and also uses ImageJ functionalities. The pipeline is affordable without knowledge in image analysis.

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