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Institute for Environmental Protection and Research (ISPRA), Rome (Italy)

3 ARTICLES PUBLISHED IN JoVE

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Biology

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana
Loredana Manfra 1, Federica Savorelli 2, Marco Pisapia 1, Erika Magaletti 1, Anna Maria Cicero 1
1Department of Environmental Quality Monitoring, Institute for Environmental Protection and Research, 2Regional Agency for Environmental Protection in Emilia-Romagna

This study concerns the development and standardization of a valuable methodological protocol to determine long-term (14 days) lethal toxicity exerted by chemical substances, industrial wastewater or sewage and liquid environmental samples on the saltwater crustacean, Artemia franciscana.

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Environment

Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants
Alice Rotini 1,2, Loredana Manfra 1,3, Filippo Spanu 2, Marco Pisapia 1, Anna Maria Cicero 1, Luciana Migliore 2
1Institute for Environmental Protection and Research (ISPRA), Rome (Italy), 2Department of Biology, Tor Vergata University, Rome (Italy), 3Department of Biology and Evolution of Marine Organisms, Stazione Zoologica, Anton Dohrn, Naples (Italy)

This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC50, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure.

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Environment

Data Collection on Marine Litter Ingestion in Sea Turtles and Thresholds for Good Environmental Status
Marco Matiddi 1, Giuseppe A. deLucia 2, Cecilia Silvestri 1, Gaëlle Darmon 3, Jesús Tomás 4, Christopher K. Pham 5, Andrea Camedda 2, Frederic Vandeperre 5,6, Françoise Claro 7, Yakup Kaska 8, Helen Kaberi 9, Ohiana Revuelta 4, Raffaella Piermarini 1, Roberto Daffina 1, Marco Pisapia 1, Daniela Genta 1, Doğan Sözbilen 8, Mohamed N. Bradai 10, Yasmina Rodríguez 5, Delphine Gambaiani 3, Catherine Tsangaris 9, Olfa Chaieb 10, Judicaëlle Moussier 7, Ana L. Loza 11, Claude Miaud 3, 13
1Italian National Institute for Environmental Protection and Research (ISPRA), 2Institute for Coastal Marine Environment-National Research Council (IAMC-CNR), 3EPHE, PSL Research University, UMR 5175 CE3FE, CNRS, UM, Univ P. Valery, SupAgro, IRD, INRA, Biogéographie et Écologie des Vertébrés, 4Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, 5Departamento de Oceanografia e Pescas, Instituto do Mar/Okeanos, Universidade dos Açores, 6MARE - Marine and Environmental Sciences Centre, Universidade dos Açores, 7Muséum national d' Histoire naturelle (MNHN), 8Sea Turtle Research and Application Center (DEKAMER), Pamukkale University, 9Institute of Oceanografy Hellenic Centre for Marine Research (HCMR), 10Institut National des Sciences et Technologies de la Mer (INSTM), 11University of Las Palmas de Gran Canaria, 12INDICIT consortium

The protocol focuses on the collection of sea turtle samples, describing all the steps from the animal recovery and necropsy to the classification and quantification of ingested marine litter. Moreover, the representative results show how to use the collected data to elaborate the possible thresholds for Good Environmental Status.

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