Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and ScreeningJavier Viña-Gonzalez 1, David Gonzalez-Perez 1, Miguel Alcalde 1
1Department of Biocatalysis, Institute of Catalysis, CSIC
We present a detailed protocol to construct and screen mutant libraries for directed evolution campaigns in Saccharomyces cerevisiae.