Jiangsu Normal University

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Cell-Free Protein Synthesis System for Building Synthetic Cells

Mengjiao Cao^*1, Mengmeng Yang^*1, Yingqiu Li^*1, Ke Yue¹, Lulu Shen¹, Lei Kai¹

¹School of Life Sciences, Jiangsu Normal University

This protocol describes the Cell-Free Protein Synthesis (CFPS) system used in constructing synthetic cells. It outlines key stages with representative results in different micro-compartments. The protocol aims to establish best practices for diverse laboratories in the synthetic cell community, advancing progress in synthetic cell development.