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King Saud University

7 ARTICLES PUBLISHED IN JoVE

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Bioengineering

20 mJ, 1 ps Yb:YAG Thin-disk Regenerative Amplifier
Ayman Alismail 1,2, Haochuan Wang 1,3, Jonathan Brons 3, Hanieh Fattahi 1,3
1Department of Physics, Ludwig Maximilian University of Munich, 2Physics and Astronomy Department, King Saud University, 3Max Planck Institute of Quantum Optics

A protocol for the operation of a high-energy, high-power optical parametric chirped pulse amplifier pump source based on an Yb:YAG thin-disk regenerative amplifier is presented here.

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Bioengineering

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
Tzu-Cheng Sung *1, Hsing-Fen Li *1, Akon Higuchi 1,2, Qing-Dong Ling 3,4, Jia-Sin Yang 1, Yeh-Chia Tseng 1, Chih-Hsien Pan Pan 1, Abdullah A. Alarfaj 2, Murugan A. Munusamy 2, Suresh Kumar 5, Shih-Tien Hsu 6, Kadarkarai Murugan 7,8
1Department of Chemical and Materials Engineering, National Central University, 2Department of Botany and Microbiology, King Saud University, 3Cathay Medical Research Institute, Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, 5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 6Department of Internal Medicine, Taiwan Landseed Hospital, 7Department of Zoology, Bharathiar University, 8Thiruvalluvar University

A protocol is presented to prepare polyvinyl alcohol-co-itaconic acid hydrogels with varying stiffness, which were grafted with and without oligopeptides, to investigate the effect of the stiffness of biomaterials on the differentiation and proliferation of stem cells. The stiffness of the hydrogels was controlled by the crosslinking time.

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Bioengineering

Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo
Alaa T Alshareeda 1, Batla Alsowayan 1, Abdullah Almubarak 2, Ayidah Alghuwainem 1, Yasser Alshawakir 2, Mohammed Alahmed 2
1Stem Cell and Regenerative Medicine Department, King Abdullah International Medical Research Centre, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, 2College of Medicine, Experimental Surgery and Animal Laboratory, King Saud University

Here, we present a protocol to develop an in vivo cancer model using cell sheet technology. Such a model could be very useful for the evaluation of anticancer therapeutics.

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Bioengineering

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
Abdulaziz S. Fakhouri 1,2,3, Jennifer L. Leight 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

Here, a protocol is presented for encapsulating and culturing cells in poly(ethylene glycol) (PEG) hydrogels functionalized with a fluorogenic matrix metalloproteinase (MMP)-degradable peptide. Cellular MMP and metabolic activity are measured directly from the hydrogel cultures using a standard microplate reader.

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Biology

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
Yunhee Ji *1, Mahsa Karbaschi *2, Abdulhadi Abdulwahed *3, Natalia S. Quinete 4, Mark D. Evans 5, Marcus S. Cooke 1
1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences, University of South Florida, 2Cepheid (Danaher Corp.), US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment, Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University

The comet assay is a popular means of detecting DNA damage. This study describes an approach to running slides in representative variants of the comet assay. This approach significantly increased the number of samples while decreasing assay run-time, the number of slide manipulations, and the risk of damage to gels.

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Bioengineering

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Sukumaran Anil 1,2, Nebu G. Thomas 2, Elna P. Chalisserry 2, Yogesh B. Dalvi 2, Ramya Ramadoss 3, Sajith Vellappally 4
1Department of Dentistry, Oral Health Institute, Hamad Medical Corporation, 2Pushpagiri Institute of Medical Sciences and Research Centre, 3Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, 4College of Applied Medical Sciences, King Saud University

This protocol emphasizes the extraction, culture, and preservation of multipotent stem cells from dental pulp through enzymatic digestion. Additionally, it demonstrates their potential to differentiate into osteoblasts, adipocytes, and chondrocytes, highlighting the importance of precision and consistency in the process.

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Chemistry

Synthesis of Black Seed Oil-loaded-, Alginate-based, pH-sensitive Beads Using Electrospraying Technique
Abul Kalam Azad 1, Mohamad Dayoob 2, Vinoth Kumarasamy 3, Vetriselvan Subramaniyan 4, Ghadeer M. Albadrani 5, Muath Q. Al-Ghadi 6, Mohamed Kamel 7, Mohamed M. Abdel-Daim 8,9, Mireia Mallandrich Miret 10, Abd Almonem Doolaanea 11,12
1Faculty of Pharmacy, University College of MAIWP International, 2Faculty of Pharmacy, MAHSA University, 3Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 4Department of Medical Sciences, School of Medical and Life Sciences, Sunway University, 5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, 6Department of Zoology, College of Science, King Saud University, 7Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 8Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, 9Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, 10Department of Pharmacy, Pharmaceutical Technology and Physical-Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 11Research and Development, Sabrena Experience, 12Alphastar Lab Systems

A technique employing high electric voltage and a targeted, active ingredient-loaded emulsion to fabricate pH-responsive, uniform microbeads is presented.

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