Process Evaluation and Kinetics of Recombinant Chitin Deacetylase Expression in E. coli Rosetta pLysS Cells Using a Statistical TechniqueGoutam Mohan Pawaskar 1, Ritu Raval 1, Subbalaxmi Selvaraj 1
1Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education (MAHE)
In the current protocol, a statistical technique, central composite design (CCD), was applied to optimize the process conditions for the expression of recombinant bacterial chitin deacetylase (BaCDA) in E. coli Rosetta pLysS cells. The employment of CCD resulted in a ~2.39-fold increase in the expression and activity of BaCDA.
