Manipal Academy of Higher Education, Manipal, Karnataka, India-576104

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

Nivedha Veerasubramanian^*1, Vaishnavi Karthikeyan^*1, Sridevi Hegde², Anandh Dhanushkodi¹, Shagufta Parveen¹

¹Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, ²Department of Medical Genetics, Manipal Hospitals

This protocol describes the generation of integration free iPSCs from fetal tissue fibroblasts through delivery of episomal plasmids by nucleofection followed by description of methods used for iPSC characterization and neuronal differentiation.