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Biology

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis
Anna K. Schellhaus *1, Adriana Magalska *2, Allana Schooley 1, Wolfram Antonin 1
1Friedrich Miescher Laboratory, Max Planck Society, 2Nencki Institute of Experimental Biology, Polish Academy of Sciences

The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.

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