JoVE Logo

Sign In

Medical University of South Carolina, College of Medicine

1 ARTICLES PUBLISHED IN JoVE

image

Developmental Biology

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
Khujista D. Haque 1, Atul K. Pandey 1, Matthew W. Kelley 2, Chandrakala Puligilla 1
1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, College of Medicine, 2Laboratory of Cochlear Development, NIDCD, NIH

We present a method that describes isolation and culture of cochlear explants from embryonic mouse inner ear. We also demonstrate a method for gene transfer into cochlear explants via square-wave electroporation. The in vitro explant culture coupled with gene transfer technique enables researchers to study the effects of altering gene expression during development.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved