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Oregon Health and Sciences University

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
Hua Wen 1, Paul Brehm 1
1Vollum Institute, Oregon Health and Sciences University

Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target skeletal muscle. This video demonstrates the microscopic methods used to identify a segmental CaP motor-neuron and target muscle cells as well as the methodologies for recording from each cell type.

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