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Pohang University of Science and Technology (POSTECH)

7 ARTICLES PUBLISHED IN JoVE

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Chemistry

Synthesis of Poly(N-isopropylacrylamide) Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
Kyoung Duck Seo 1, Andrew Choi 1, Junsang Doh 1,2, Dong Sung Kim 1
1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 2School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH)

We present a protocol to synthesize Janus microhydrogels composed entirely of the same base material, poly(N-isopropylacrylamide) (PNIPAAm), with a clearly compartmentalized structure base on the phase separation of a supersaturated NIPAAm monomer solution. The synthesized Janus microhydrogels show unique properties such as anisotropic thermo-responsiveness and organophilic/hydrophilic loading capability.

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Engineering

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
Dasol Lee *1, Minkyung Kim *1, Sunae So *1, Inki Kim 1, Gwanho Yoon 1, Kyunghoon Kim 2, Junsuk Rho 1,3
1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 2School of Mechanical Engineering, Sungkyunkwan University, 3Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH)

The use of a hyperlens has been regarded as a novel super-resolution imaging technique due to its advantages in real-time imaging and its simple implementation with conventional optics. Here, we present a protocol describing the fabrication and imaging applications of a spherical hyperlens.

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Engineering

Demonstration of Equal-Intensity Beam Generation by Dielectric Metasurfaces
Gwanho Yoon 1, Dasol Lee 1, Junsuk Rho 1,2,3
1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 2Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), 3National Institute of Nanomaterials Technology (NINT)

A protocol for the fabrication and optical characterization of dielectric metasurfaces is presented. This method can be applied to the fabrication of not only beam splitters, but also of general dielectric metasurfaces, such as lenses, holograms, and optical cloaks.

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Bioengineering

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
Jaewook Kim *1, Myungji Kim *2, Dong Gyu Hwang 2, In Kyong Shim 3, Song Cheol Kim 3,4, Jinah Jang 1,2,5
1Department of Mechanical Engineering, Pohang University of Science and Technology, 2School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 3Asan Institute for Life Science, University of Ulsan College of Medicine and Asan Medical Center, 4Division of Hepato-Biliary and Pancreatic Surgery, Department of Surgery, University of Ulsan College of Medicine and Asan Medical Center, 5Department of Creative IT Engineering, Pohang University of Science and Technology

Decellularized extracellular matrix (dECM) can provide suitable microenvironmental cues to recapitulate the inherent functions of target tissues in an engineered construct. This article elucidates the protocols for the decellularization of pancreatic tissue, evaluation of pancreatic tissue-derived dECM bioink, and generation of 3D pancreatic tissue constructs using a bioprinting technique.

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Engineering

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms
Inki Kim 1, Dasol Lee 1, Junsuk Rho 1,2
1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 2Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH)

We present a protocol for fabrication of spin- and direction-multiplexed visible metaholograms, then conduct an optical experiment to verify their function. These metaholograms can easily visualize encoded information, so they can be used for projective volumetric display and information encryption.

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Bioengineering

3D Cell-Printed Hypoxic Cancer-on-a-Chip for Recapitulating Pathologic Progression of Solid Cancer
Wonbin Park *1, Mihyeon Bae *1, Minseon Hwang 1, Jinah Jang 2, Dong-Woo Cho 1, Hee-Gyeong Yi 1,3
1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 2Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), 3Department of Rural and Biosystems Engineering, College of Agriculture and Life Sciences, Chonnam National University

Hypoxia is a hallmark of tumor microenvironment and plays a crucial role in cancer progression. This article describes the fabrication process of a hypoxic cancer-on-a-chip based on 3D cell-printing technology to recapitulate a hypoxia-related pathology of cancer.

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Biochemistry

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
Celine Park *1, Taehyun Yang *1, Sang-Hyun Rah 1, Hyun Gyu Kim 2,3, Tae-Young Yoon 2,3, Min Ju Shon 1,4
1Department of Physics, Pohang University of Science and Technology (POSTECH), 2School of Biological Sciences, Seoul National University, 3Institute for Molecular Biology and Genetics, Seoul National University, 4School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH)

Here, we describe a high-speed magnetic tweezer setup that performs nanomechanical measurements on force-sensitive biomolecules at the maximum rate of 1.2 kHz. We introduce its application to DNA hairpins and SNARE complexes as model systems, but it will be also applicable to other molecules involved in mechanobiological events.

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