Providence College

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Quantitatively Measuring In situ Flows using a Self-Contained Underwater Velocimetry Apparatus (SCUVA)

Kakani Katija¹, Sean P. Colin^2,3, John H. Costello^3,4, John O. Dabiri⁵

¹Applied Ocean Physics and Engineering, Woods Hole Oceanographic Institution, ²Environmental Science and Marine Biology, Roger Williams University, ³Marine Biology Laboratory, Whitman Center, ⁴Department of Biology, Providence College, ⁵Departments of Aeronautics and Bioengineering, California Institute of Technology

This protocol provides instructions on how to use a self-contained underwater velocimetry apparatus (SCUVA), which is designed for quantification of in situ animal-generated flows. In addition, this protocol addresses challenges posed by field conditions, and includes operator motion, predicting position of animals, and orientation of SCUVA.

Behavior

Testing for Metacognitive Responding Using an Odor-based Delayed Match-to-Sample Test in Rats

Keith A. Lee¹, Aidan J. Preston¹, Taylor B. Wise¹, Victoria L. Templer¹

¹Department of Psychology, Providence College

This protocol describes a method for investigating the possibility of metamemory, or memory awareness, in rodents. The odor-based delayed-matching-to-sample paradigm is a novel, ecologically-relevant behavioral test useful for determining the extent to which rodents can adaptively respond based on cognitively monitoring the strength of their memory states.

Neuroscience

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

Kathryn E. Neville¹, Timothy L. Bosse¹, Mia Klekos¹, John F. Mills¹, Marla Tipping¹

¹Department of Biology, Providence College

We present a protocol for measuring oxygen consumption and extracellular acidification in Drosophila melanogaster larval and adult brains. A metabolic analyzer is utilized with an adapted and optimized protocol. Micro-tissue restraints are a critical component of this protocol and were designed and created specifically for their use in this analysis.